FastQCFastQC Report
Sun 14 Apr 2019
SRR6575899_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575899_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1029652
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA25980.252318258984589No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT24320.2361963071018169No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC21730.21104217735700995No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT17060.16568704766270545No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC13980.13577402850671877No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13580.1318892208241231No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT12460.12101175931285522No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA11940.11596150932548085No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG11750.11411622567624789No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11030.10712357184757568No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA10690.10382148531736936No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10530.10226756224433109No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10510.10207332186020131No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC10470.10168484109194176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGTAC5700.029.6852666
GGGTACC5700.028.8578747
ATGGGTA6100.028.509215
TAGTACT9000.028.2010064
CCTAGAC1005.3217092E-528.2010043
ATTAGAC1853.274181E-1027.9469433
CATGGGG28050.026.9777894
CGTTAAC1755.5551936E-926.8554929
CATGGGT7900.026.7731084
GTATAAG4950.026.6645031
GGTACCT6000.026.6316958
GACGTTA1851.00408215E-825.4038457
CCTAGTA10750.024.9218182
CTAGTAC10750.024.9218183
TGTTAGG1151.376739E-424.5226142
GTACTGT11150.024.4493036
CGCAAGT5000.024.43968676-77
GTGATCG5000.024.43858
CTAACGC3300.024.2129843
AGGGCTA5250.024.1722895
Produced by FastQC (version 0.11.2)