FastQCFastQC Report
Sun 14 Apr 2019
SRR6575899_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575899_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1029652
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA27130.2634870810720515No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT24390.23687614844627117No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC23030.2236678023254459No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC17170.16675536977541927No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT16880.1639388842055374No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14510.14092139868615805No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG14340.1392703554210549No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT14250.13839627369247087No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA12820.12450808622719131No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12710.12343976411447752No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12400.12042903816046586No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11870.11528166798102658No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA11770.11431046606037767No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA11310.10984293722539266No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA10830.10518116800627786No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC10830.10518116800627786No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA10800.10488980743008317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG5400.035.6914561
GACGTTA1703.6379788E-1233.1758547
CTATTGA5050.031.6429779
AGGTATA1859.094947E-1230.4859186
TAACCAC2300.028.6081625
TAGTACT8250.028.484324
AGGGCTA5750.027.7907875
GTTAATA4450.027.4654981
CATGGGG28150.027.3813744
ACTATCC700.008836403526.8566448
GGCTATT5950.026.8566447
CTTAGGT2450.026.8566423
CTAACTG2650.026.603284
GGGCTAT6200.026.5317656
TATAGAC1606.915798E-826.4370083
CTAGGCA8300.026.0477094
TAGGCAT7400.026.0400545
CTAACGC3250.026.0302853
GTATAAG4350.025.935591
ATGGGGG16900.025.5853235
Produced by FastQC (version 0.11.2)