FastQCFastQC Report
Sun 14 Apr 2019
SRR6575898_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575898_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927703
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA26100.2813400409398266No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC23550.2538527955606482No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA22890.24673844969780198No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC20660.2227005841309126No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20470.22065251486736598No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC13500.1455207108309448No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG9770.10531387739395044No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT9640.103912566845208No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT9380.10110994574772314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGTA2100.033.561735
CTTAGGT5950.032.3771973
TAGTACT5550.032.170884
GTACTAG4050.031.3361021
TAGGTAT5850.031.3242785
CTGTGCG6350.030.3376879
AGGTATA6050.029.512136
CATGGGG31400.029.3291024
CGGGCGT4250.028.7446336
ATACGGG4250.028.7446333
TTAGGTA6400.027.8981864
TACGGGC4400.027.7647024
GGCGTCG4450.027.4527388
AATAGTA2950.027.076925
ATGGGGG15800.027.0617985
ATTTAGG3600.026.1134191
GTACTGT7800.025.902776
GTGTTAT1301.086788E-525.3099271
GTACATG66850.025.1720071
TCTTAGG7900.024.989552
Produced by FastQC (version 0.11.2)