FastQCFastQC Report
Sun 14 Apr 2019
SRR6575896_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575896_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658828
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA14120.21431997425731755No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT13290.2017218454589058No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8870.13463301499025543No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8360.12689199609002652No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8070.12249024024479833No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT7700.11687420692502444No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7560.11474922134456944No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7430.11277602044843266No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG7230.1097403267620684No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA7220.10958854207775018No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC7190.10913318802479556No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA7100.10776712586593162No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT6870.10427607812661271No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA6650.10093681507161202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAAG2800.031.9165691
CGTTAAC953.7308968E-529.706281
GTACTAG1602.1136657E-929.396841
TAGGTAT1303.3306424E-728.9204165
CTAAGCG650.00615505328.9204164
TAGTACT2850.028.0325074
CCTAGTA2950.027.0822532
TAATGCA700.00881115926.8709879
ATGGGAG4200.026.8546715
CTAGTAC3000.026.6308823
TAACCCT1258.022849E-626.317584
ATGGGGG8600.025.6836815
CCTAAGA3700.025.4030672
TTAGGTA1701.2432793E-724.8800624
TAGTGCA950.001300083524.7495949
GTCCTAC2101.4151738E-924.6373521
CATGGGG16850.024.5437364
GTATATA1151.3694105E-424.539971
ACATGGG37700.024.4327663
TACATGG40100.023.9080242
Produced by FastQC (version 0.11.2)