FastQCFastQC Report
Sun 14 Apr 2019
SRR6575894_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575894_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences776117
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA18690.24081420713629517No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT17960.23140840878372718No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC15270.19674868608727808No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT13530.1743293859044448No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC11580.14920430811333857No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11050.14237544081626868No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA10620.1368350390469478No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC10320.1329696424637007No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG10150.13077925106652735No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9830.12665616137773042No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT9330.12021383373898523No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG9310.11995614063343542No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA9230.1189253682112362No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9020.11621959060296322No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT8820.11364265954746514No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8800.11338496644191533No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA8590.11067918883364235No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA8020.10333493532547285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG4950.042.791981
TAGTACT5400.033.9410934
GTACTGT6200.031.835576
CGTTAAC1901.2732926E-1129.7291661
TAGGCAT7600.029.681285
CCTAGTA6750.029.2415582
CTAGTAC6750.029.2415583
CATTACT650.00615592128.9202234
ACCTAAG2300.028.6520211
TGGGTAC3950.028.5541446
GGGTACC3950.027.3626257
CTGTGCG7450.027.1388289
TACTGTG7150.026.9466537
GCGCTTA350.00884121326.85449278-79
TAGTATG700.00884131626.8527627
ACTGTGC8450.026.7093568
TAACAGT2500.026.3174024
TACTAGG8250.026.2034742
GCGTTAG551.0141234E-425.6528472-73
CTAGGCA9200.025.5409564
Produced by FastQC (version 0.11.2)