FastQCFastQC Report
Sun 14 Apr 2019
SRR6575891_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575891_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1551384
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC31720.20446259597881633No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA31020.1999504958153494No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC28810.18570515101354662No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG21930.14135765226404293No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA19720.12711230746224017No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT19640.12659663887212966No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC19300.12440504736416No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA18490.11918390288929112No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT18280.11783027284025102No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA17020.10970849254601053No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16210.10448734807114164No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT15930.10268250800575486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28850.050.0253221
TCAACGC35450.039.4991154
ATCAACG35650.039.2775233
CAACGCA36500.038.105375
AACGCAG38050.037.0470776
ACCTCGC902.5990194E-531.3253618
GGTATCA13100.031.2208921
TATCAAC45550.031.1695042
TTAGGTA7950.029.5522254
ACGCAGA47750.029.4228787
CTAAGAC14700.028.768193
GTCTTAG10450.028.3414211
TAGGTAT8550.028.0279545
CGCAGAG50600.027.765668
GTACATG65900.027.7499081
CTTAGGT8500.027.0872233
TACATGG67000.027.0145232
ACCTAAG12900.026.967421
CGTATAG700.008827087526.8632981
AGGTATA8950.026.7753096
Produced by FastQC (version 0.11.2)