FastQCFastQC Report
Sun 14 Apr 2019
SRR6575890_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575890_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1765813
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA34370.19464122191874222No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC33840.1916397715952935No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC28590.16190842405169745No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC20000.11326227635655645No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG20000.11326227635655645No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT19550.11071387513853392No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT18600.10533391701159749No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA18220.10318193376082291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29750.049.1198651
TCAACGC37850.037.5009774
ATCAACG38800.036.8250543
CAACGCA38900.036.2460675
AACGCAG39500.035.5765086
GGTATCA12700.033.405751
TATCAAC48500.030.0414892
TAGGTAT9850.029.5831685
GTCTTAG12100.029.218531
CTTAGGT10150.028.2465463
GTACATG71550.028.0002381
TACATGG71150.027.678382
TTAGGTA10250.027.5124244
ACGCAGA51300.027.3000567
ACATGGG73050.027.0228163
CGCAGAG54100.025.9739848
AGGTATA10700.025.915376
ACCTAAG14300.025.382661
TCTTAGG13100.025.1147162
CTAAGAC16500.024.2123263
Produced by FastQC (version 0.11.2)