FastQCFastQC Report
Sun 14 Apr 2019
SRR6575888_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575888_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1268669
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG18650.14700445900388517No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA16210.12777170404573612No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC14290.11263773293112703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25950.055.3989451
TCAACGC31750.045.294794
ATCAACG32800.043.1317833
CAACGCA33000.043.009425
AACGCAG33800.042.1304866
GGTATCA9500.039.1960641
TATCAAC39500.036.172732
ACGCAGA42750.033.0903137
CGCAGAG44800.031.5761388
GTACTAG600.004103342531.423011
GTACATG60800.029.3040221
TACATGG61000.029.0480022
GCAGAGT49550.028.5491649
GTCTTAG6150.028.357351
ACATGGG62200.028.26093
TAGGTAT4400.027.770985
CTTAGGT4500.026.1115263
TTAGGTA5000.025.3784054
AGGTATA4650.025.2672276
GGGGATA3200.024.9671785
Produced by FastQC (version 0.11.2)