FastQCFastQC Report
Sun 14 Apr 2019
SRR6575881_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575881_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325625
Sequences flagged as poor quality0
Sequence length100
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6270.192552783109405No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4100.12591170825335893No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3760.11547024952015354No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3590.11024952015355086No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3420.10502879078694818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8750.052.647051
ATGGGTA3050.038.5238345
ATCAACG12650.036.4103553
TCAACGC13000.035.7916074
CAACGCA12900.035.7047235
AACGCAG13100.035.5183876
CATATAG959.4244933E-734.6308983
CATGGGT5300.034.584234
TACATGG21200.034.3678132
ACATGGG20800.034.345483
GTACATG21150.034.2268071
TGGGTAC3200.033.780596
GACATAT702.1798613E-433.5759281
GGGTACC3400.031.7934957
ACGCAGA14400.031.6591037
CGCAGAG14950.030.4943858
TATCAAC15500.030.0233762
GGTACCT3850.029.298138
ATGGGAG4250.028.7523785
CATGGGA8900.028.5162944
Produced by FastQC (version 0.11.2)