FastQCFastQC Report
Sun 14 Apr 2019
SRR6575878_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575878_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences761757
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA26120.34289149952018816No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT23720.3113853893039381No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC18090.23747730575498488No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC14310.187855182164391No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG13880.18221033741731288No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT13410.17604039083329723No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT11280.1480787180163753No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA10930.14348407694317217No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA10670.1400709150030784No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC9600.12602444086500025No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA9430.12379275805801589No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC9360.12287382984337526No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT9330.12248000346567212No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA8970.11775408693323461No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG8880.11657260780012524No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8530.1119779667269221No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT8480.11132158943075023No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8180.10738332565371897No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT8130.10672694835754709No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG8120.10659567289831272No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC7840.10291996003975021No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7790.10226358274357833No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT7750.10173848090664084No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTATCG250.00524135856.3836948
GGACCGT652.7892238E-643.3720746
TAGGTAT1450.038.8853075
CTACCCC757.451712E-637.59164
TTAGGTA1750.034.9064834
ACGTTAA1107.7101504E-834.1719368
GACGTTA1107.7101504E-834.1719367
AGGTATA1755.456968E-1232.2192546
GTATAAG4850.031.9827351
TAGTACT6350.031.819664
TCTTAGG2000.030.55322
CTTAGGT1859.094947E-1230.4796753
GTACTGT7150.030.2290216
ACTATAT3750.030.073283
TCTAACG3000.029.7697872
CTAACTG3000.029.7600144
TAGTATG953.75804E-529.6756297
CTAACGC2900.029.1658953
GTACTAG5200.028.9261071
TAGACCT650.00615960428.9166134
Produced by FastQC (version 0.11.2)