FastQCFastQC Report
Sun 14 Apr 2019
SRR6575877_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575877_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820757
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21790.26548661784182165No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20710.252328033754205No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC19850.24184990198073245No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT15420.18787533947319365No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC13380.16302023619658437No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG12620.15376049183863189No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC11290.13755593921221507No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT11230.13682490676290301No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA11070.1348754868980709No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA10780.13134216339306276No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA10000.12183874155200626No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9590.116843353148374No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT8920.1086801574643896No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA8600.10478131773472538No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC8470.10319741409454931No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTAGG2550.033.1778032
CTTAGGT2000.032.901323
GTACTAG6050.032.7251171
TAGGTAT1900.032.1591875
GACGTTA1452.660272E-829.1700337
CTAACGC3550.029.1279323
TTAGGTA2101.8189894E-1229.0964094
AGGTATA2101.8189894E-1229.0964096
TCTAACG3400.029.030582
TAGTACT7250.028.5252844
ACGCCTA3850.026.8582236
TAGGCAT8450.026.6992975
GTACTGT8150.026.5286736
TATAAAA10100.026.0604522
CATGGGG22350.025.4462134
TCAAGAC1851.0024451E-825.4064273
ATCAGGC10000.025.3779288
CTAGTAC9300.025.2698333
ATGGGGG13350.024.6451855
ATAAAAT9950.024.56383
Produced by FastQC (version 0.11.2)