FastQCFastQC Report
Sun 14 Apr 2019
SRR6575875_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575875_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666636
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20110.3016638765383208No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20060.3009138420367337No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC16240.24361120611548132No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA15250.22876052298405727No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC13360.2004092188240659No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT11610.17415801126851838No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT10890.1633575144456645No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC9670.1450566726069399No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC9250.1387563827936085No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC9210.13815635519233885No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA8910.1336561481828164No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT8750.13125603777773776No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG8670.13005598257519846No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA7910.11865545815107495No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT7850.11775541674917046No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT7190.1078549613282211No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG7180.10770495442790368No Hit
ATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCA7110.10665490612568178No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG6900.10350476121901608No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT6820.10230470601647675No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC6720.10080463701330261No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTACG503.0088218E-546.9979794
TTAGGTA1103.8198777E-1142.7318424
TAGGTAT952.1125743E-839.583185
ACCTAAG2150.039.4566461
CCTAAGA2450.036.4528772
CTTAGGT1204.1964086E-935.253773
GTACTAG4700.032.0876731
AGGGCTA3200.030.8470485
CATGGGG17750.030.1891444
ATGGGGG11900.029.6250175
GTATATA1303.2492972E-729.002321
TAAGGGT650.006149125328.9261684
ACCGTGC650.006153634328.9218318
GTACTTG2002.5465852E-1128.2772621
AGGTATA1354.6254536E-727.854836
GGGCTAT3450.027.249296
TAGGCAT6450.026.9641235
GTTCGGG700.00871631226.9307251
CTATTGA3400.026.2635739
ACAGACT3050.026.1955918
Produced by FastQC (version 0.11.2)