FastQCFastQC Report
Sun 14 Apr 2019
SRR6575869_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575869_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences989691
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA21540.21764368878771254No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC17130.17308432632003323No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC12190.12316975702517251No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11240.11357080139154543No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA10310.10417392903441579No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10280.10387080411966969No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10210.10316351265192873No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10010.10114267988695462No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9930.10033434678096496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26300.039.1873551
GACGTTA651.4148232E-436.147437
TCAACGC33450.030.9063244
GGGTACC5950.030.801267
CAACGCA33150.030.760765
ATCAACG34000.030.406373
AACGCAG34750.030.0205756
GTACATG61850.029.8266141
AGGTATA3500.029.5376176
TACATGG63900.028.9024372
ACATGGG62300.028.8889373
TGGGTAC6200.028.801346
GTACTTG6550.028.7393071
GGTACCT6750.028.5632888
TATCAAC37350.027.8063772
GTCTTAG5850.027.3514691
TTAGGTA4150.027.1759034
ACCTAAG8900.026.967321
TAGGTAT3900.026.5081185
CTTAGGT4300.026.2279053
Produced by FastQC (version 0.11.2)