FastQCFastQC Report
Sun 14 Apr 2019
SRR6575869_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575869_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences989691
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA20000.2020832764974118No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16960.17136661846980522No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC12900.1303437133408306No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12450.12579683961963886No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12360.1248874648754005No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11840.11963329968646778No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10520.10629580343763861No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10450.10558851196989767No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10120.10225413790769039No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31000.033.813951
AGGTATA4450.030.6207036
GTACATG62200.030.0777531
TACATGG63650.029.2448582
GTCTTAG7400.029.2199081
ACATGGG62650.028.8746573
TAGGTAT5050.028.8434685
CAACGCA37200.027.9142845
CATGGGG26100.027.9041214
TCAACGC37400.027.7650094
ATCAACG37550.027.6540993
AACGCAG38750.027.282746
TTAGGTA5250.026.849684
CTTAGGT5450.026.7265173
GGGTACC5700.026.3786337
ACCTAAG7550.026.1489981
GGTACCT5950.026.0599848
CCCTATA1659.303585E-825.6396032
ATGGGAG10900.024.5711545
TATCAAC42950.024.5153222
Produced by FastQC (version 0.11.2)