FastQCFastQC Report
Sun 14 Apr 2019
SRR6575866_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575866_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1439372
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA21310.1480506776566447No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC19070.1324883351906248No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16860.11713441695406053No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC16630.1155364978615674No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA16030.11136801327245494No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15690.10900587200529119No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA14440.10032152911130687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20950.056.3252261
GGTATCA7750.043.0695081
TCAACGC27550.042.3065454
ATCAACG27600.042.22993
CAACGCA28700.040.775095
AACGCAG30250.039.1518826
ACGTATA500.001702562237.5982367
TATCAAC36200.032.8567962
ACGCAGA37250.031.6683127
TTAGGTA6450.031.3318634
TAGGTAT6250.030.8305555
GTACATG54000.030.8193021
TACATGG53750.030.3503912
CTTAGGT6200.029.5631283
GTCTTAG8700.029.1801661
ACATGGG55400.028.843413
AGGTATA6550.028.7009456
CGCAGAG40950.028.6921838
GGTACCT7000.027.5272798
GCAGAGT44850.026.5115769
Produced by FastQC (version 0.11.2)