FastQCFastQC Report
Sun 14 Apr 2019
SRR6575865_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575865_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367942
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC6410.17421223997260438No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA5450.1481211712715591No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC4240.11523555342961662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2450.048.0032651
GTATCAA6200.047.801961
TCAACGC7550.037.3446734
ATCAACG7700.037.2274673
CAACGCA7650.036.2422335
GGTAATC550.002707829234.1992768
AACGCAG8500.033.723716
TATAGGG702.1842959E-433.565752
GTACATG12800.033.4447751
ACATGGG14050.031.7739833
TACATGG13850.031.2149352
TATCAAC9650.030.1917842
CATGGGG8100.030.1677344
ACGCAGA9600.028.8805317
GTATAGG1005.280658E-528.225921
GGCGCGA505.297565E-528.2182470-71
TATAAGG1005.3199663E-528.195232
CGCAGAG10150.027.7974418
TATATGC856.813954E-427.6423822
TAGGCGG856.813954E-427.6423825
Produced by FastQC (version 0.11.2)