FastQCFastQC Report
Sun 14 Apr 2019
SRR6575864_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575864_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455727
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA6850.15030928604186278No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC5950.13056062072249394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6650.051.0014081
GGTATCA2750.046.249011
ATCAACG9050.034.7971233
TCAACGC9150.033.9031454
CAACGCA9400.033.0014655
GTACATG14950.032.4539341
ACATGGG15700.032.3326453
TACATGG16050.031.3347232
AACGCAG9950.030.2324966
GGTACCT2051.8189894E-1229.8029298
TATCAAC10900.029.7536142
CATGGGG9200.028.6099644
GTATTAC1057.299675E-526.9174081
ATTATCC4850.026.1661073
CTATTAT5700.024.7923491
GTACGGC950.001303041124.737946
TTATCCT5600.024.3403644
ACGCAGA12650.023.7771027
TATTATC5350.023.7206762
TATCCTG5200.023.5010415
Produced by FastQC (version 0.11.2)