FastQCFastQC Report
Sun 14 Apr 2019
SRR6575864_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575864_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455727
Sequences flagged as poor quality0
Sequence length100
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC8220.18037114325023534No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA6870.15074814527118208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6550.061.0028341
TCAACGC8400.045.319264
CAACGCA8950.043.059395
ATCAACG8800.042.725233
AACGCAG9200.041.88936
TATCAAC10750.035.8573152
ACGCAGA11350.034.7824757
GTATAGG550.002713011334.1876871
GTACATG16950.032.725381
TATAGGG600.004154878731.338712
TCTATAC902.5913638E-531.3318353
CGCAGAG12800.031.2094448
TACATGG17500.030.3537792
ACATGGG18150.029.5192493
AGGTATA1952.0008883E-1128.9216926
ACCTAAG3150.028.354071
TAGGTAT2002.7284841E-1128.198655
GCAGAGT14550.027.7787389
GTCTTAG2950.027.0893961
GGTATCA1755.524271E-926.8617521
Produced by FastQC (version 0.11.2)