FastQCFastQC Report
Sun 14 Apr 2019
SRR6575863_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575863_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1435413
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17480.12177679873318689No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16520.115088828093378No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15550.10833119109273778No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT14940.10408154308202587No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14910.10387254399953184No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14540.10129488864877216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19750.057.9278561
GGTATCA7600.044.6033251
ATCAACG25350.044.1209343
TCAACGC25700.043.885784
CAACGCA26600.042.224255
AACGCAG27750.040.8116956
TATCAAC33900.033.6909142
ACGCAGA34950.032.135227
GTACATG51950.030.5416621
TACATGG51900.030.5188922
CGCAGAG38600.029.359458
ACATGGG53950.029.1809273
GTCTTAG6550.026.5955351
GCAGAGT42800.026.0389049
TAGGTAT4650.025.2657835
CGTATAG950.001291669424.7796251
CTTAGGT4950.024.6839033
TTAGGTA5000.024.4370654
CATGGGG33550.023.672274
TGGGTAC9700.023.2541286
Produced by FastQC (version 0.11.2)