FastQCFastQC Report
Sun 14 Apr 2019
SRR6575863_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575863_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1435413
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA18030.12560844857891074No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17350.12087113604237945No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17240.12010480607323468No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16880.11759681708330633No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA16150.11251117274261833No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC16100.11216284093846163No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA15250.10624120026779749No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19450.055.1018561
ATCAACG25800.040.2477843
TCAACGC26350.039.40774
CAACGCA27650.037.7248275
AACGCAG29200.035.722316
GGTATCA8200.035.5409361
TATCAAC35150.029.6878432
GTACATG54200.029.6604561
TACATGG53650.029.5264432
ACCTAAG6100.029.2822691
ACGCAGA36800.028.3448777
ACATGGG56500.027.5263713
CGCAGAG39900.026.1426438
GCAGAGT45100.023.3367779
CTAAGAC9100.023.2348843
TAAGACA9550.023.1240484
TTATGGG2853.6379788E-1223.080894
CATGGGG35350.022.8616944
AGAGTAC37300.022.6742610-11
TACGTGT4250.022.1111074
Produced by FastQC (version 0.11.2)