FastQCFastQC Report
Sun 14 Apr 2019
SRR6575862_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575862_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1571037
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17430.11094582750119826No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17410.1108185230519714No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16230.10330756054758734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20550.054.591341
GGTATCA8500.041.5911521
ATCAACG26850.040.9596863
TCAACGC27100.040.407124
CAACGCA28050.038.535965
AACGCAG30250.036.1994366
TATCAAC35400.031.7307032
GTACATG57700.030.2262691
TACATGG58400.029.8569982
ACATGGG58550.029.138343
ACGCAGA39550.027.6873057
CGCAGAG42350.025.8567398
GGTACCT10650.024.7121018
GGGTACC11300.024.1224167
GCAGAGT46800.023.4985629
CATGGGG36500.023.1766644
ATGGGTA11850.022.6062095
GTCTTAG6750.022.3462581
ACCTAAG5850.021.7553731
CTATTAA4350.021.6720181
Produced by FastQC (version 0.11.2)