FastQCFastQC Report
Sun 14 Apr 2019
SRR6575862_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575862_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1571037
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA18680.11890235557787626No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT18470.11756565886099435No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG18140.11546513544875137No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA17770.11311000313805467No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA16990.10814512961820757No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16960.10795417294436732No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC16040.10209816827993229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23200.050.8621061
GGTATCA10700.039.982161
ATCAACG29950.039.230823
TCAACGC30400.038.6500974
CAACGCA31650.037.420625
AACGCAG32650.036.274516
GTACATG59300.028.540161
TATCAAC41150.028.447112
ACGCAGA41950.028.232727
TACATGG59500.028.128182
ACCTAAG6400.027.9133661
ACATGGG61050.027.0212613
CGCAGAG44550.026.6905178
TAAGACA10100.025.5932544
CTAAGAC9250.024.388423
GTATACG1351.4550073E-524.3765851
GGTACCT9700.024.2260428
GCAGAGT49900.023.9230969
ATGGGTA11250.023.3948215
AGAGTAC41500.022.70651210-11
Produced by FastQC (version 0.11.2)