FastQCFastQC Report
Sun 14 Apr 2019
SRR6575861_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575861_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1230956
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA29870.2426569268113564No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC23660.19220833238556048No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC22930.1862779823161835No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16910.1373729036618693No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15490.12583715421184835No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT14480.11763214932134049No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14300.11616987122204205No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14270.1159261582054923No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14240.11568244518894256No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13370.10861476770900017No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG12680.10300936832835617No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT12680.10300936832835617No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT12330.10016604980194255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19500.052.8634381
GGTATCA8650.040.2681351
ATCAACG25350.039.298863
TCAACGC25500.039.0676884
CAACGCA26200.037.665185
AACGCAG27300.035.9768646
TATCAAC32200.031.5224462
ACCGATC600.00415059631.3495128
TACCGAT753.2782494E-431.3291367
GTCTTAG9300.030.8739931
TTAGGTA7450.030.2765944
ACCTAAG12100.029.5647451
TAGGTAT7350.029.4098325
ACGCAGA33750.028.9620467
GTACATG49750.028.8570981
AGGTATA7550.028.6319246
TACATGG50200.028.1763532
TAAGACA14300.027.9321844
CTAAGAC13550.027.7442343
ACCAGAT12800.027.55664394
Produced by FastQC (version 0.11.2)