FastQCFastQC Report
Sun 14 Apr 2019
SRR6575861_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575861_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1230956
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA26550.21568601964651865No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC22240.18067258293553953No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC21950.1783166904422254No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16200.13160502893685883No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15770.12811180903297925No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15600.12673076860586405No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA15190.12340002404635096No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14690.11933814043718866No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA14160.11503254381147661No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14140.11487006846711012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19700.048.428521
TCAACGC24800.038.0826874
CAACGCA25300.037.7015045
ATCAACG25100.037.6275143
AACGCAG27000.035.5017366
GGTATCA8300.035.1062931
GTCTTAG7750.029.714321
TATCAAC32550.029.453232
GTACATG52700.028.9830531
TACATGG53400.028.6019652
ACATGGG53900.028.3320353
ACGCAGA34250.027.9867697
ACCTAAG10050.027.5903591
AGGTATA7050.027.3261176
GGTACCT7750.026.6768268
TAGGTAT7250.026.5722925
CTAAGAC12000.026.234743
TGGGTAC8750.025.7760496
TTAGGTA7500.025.686554
CTTAGGT7700.025.0193653
Produced by FastQC (version 0.11.2)