FastQCFastQC Report
Sun 14 Apr 2019
SRR6575860_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575860_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1368479
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA29390.21476398249443363No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC23330.17048124231354667No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC23100.16880054425387603No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17370.126929240419473No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16960.12393321344353841No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15550.11362980359947066No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15460.11297213914133868No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT15170.11085299810958005No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14370.10500709181507353No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT14250.10413020587089754No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14230.10398405821353487No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG13920.10171876952441361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20900.054.113511
GGTATCA7500.047.752171
ATCAACG26850.040.9637763
TCAACGC27150.040.5096554
CAACGCA27800.039.731565
AACGCAG29300.037.697526
TTAGGTA7900.034.50754
TAGGTAT7500.034.467835
GTCTTAG9950.033.6260641
CTTAGGT7750.033.3571853
TATCAAC35450.031.4239312
AGGTATA8200.030.9522676
GTACATG57200.030.8941331
TACATGG57900.030.4425562
GGTATAG8400.030.2119947
ACGCAGA36850.029.970597
TCTTAGG10100.029.7842752
GTACTAA953.6859936E-529.7624321
ACATGGG59450.029.3325943
CGCAGAG39050.028.5228068
Produced by FastQC (version 0.11.2)