FastQCFastQC Report
Sun 14 Apr 2019
SRR6575859_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575859_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338434
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5940.1755142804800936No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5610.1657634871200884No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4970.14685285757341165No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3410.10075819805338707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14100.033.0674171
GGTACCT2200.032.0444958
CATGGGT4650.031.3370294
ATCACGT600.00415747931.3323978
GTACATG24550.027.4327131
AACGCAG17850.027.1236446
TACATGG24700.027.023432
ACATGGG24700.027.023433
GTATTAC700.008739931526.912011
ATGGGTA2800.026.860315
ATCAACG17850.026.8603083
ACGTATT350.00883478826.8563488-89
TGGGTAC2650.026.606916
CAACGCA18400.026.3128855
TCAACGC18250.026.2715914
GGGTACC2651.8189894E-1224.8294477
ATTATCC1151.3733059E-424.5246323
TATCAAC19500.024.346462
ACGCAGA20750.023.7824237
CGCAGAG21100.023.610678
Produced by FastQC (version 0.11.2)