FastQCFastQC Report
Sun 14 Apr 2019
SRR6575859_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575859_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338434
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5710.16871827298675665No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5330.15749008669341732No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4640.13710206421340645No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA651.4115137E-436.153281
GTATCAA15800.035.695641
AACGCAG19200.031.332846
CATGGGT4850.031.0098254
CATGGGG7850.030.534554
ATCAACG19250.030.0306973
GTACATG26400.029.9086191
TACATGG25850.029.817712
TCAACGC19500.029.6456874
CAACGCA19600.029.4944325
GCAAACG650.006148588428.9226231
ACATGGG26850.028.5321433
ACGCAGA20950.028.49127
CGCAGAG21350.028.1775448
GGGTACC2750.027.3450247
ATGGGTA3100.027.2898945
TATCAAC21200.027.2684382
TAGGTGC700.0088280526.8567226
TCGCTAT350.00883417926.85672270-71
GGTACCT2900.025.9306268
Produced by FastQC (version 0.11.2)