FastQCFastQC Report
Sun 14 Apr 2019
SRR6575856_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575856_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740024
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC11060.14945461228284487No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA9990.13499562176361848No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9260.12513107682994065No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8790.11877993146168232No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7950.10742894825032702No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7940.10729381749781089No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC7870.10634790223019795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11600.049.8285031
TCAACGC14700.038.998144
ATCAACG14950.038.0316853
CAACGCA15100.037.9650765
AACGCAG15350.037.3467526
GGTATCA4600.033.712181
GTACATG27800.030.765031
ACGCAGA18700.030.4050127
CTTAGGT3400.030.405013
TATCAAC19100.030.2643832
TACATGG28650.029.5262262
ACATGGG28350.029.5031453
GTATACC804.7854756E-429.3684753
AGGTATA3250.028.9166536
GTCTTAG4300.028.4142131
GAGTATA856.8199384E-427.6427841
CGCAGAG20650.027.5338348
TAGGTAT3450.027.2403245
GGTATAG3750.026.3141567
CATGGGG17350.025.9999874
Produced by FastQC (version 0.11.2)