FastQCFastQC Report
Sun 14 Apr 2019
SRR6575855_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575855_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences795304
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC12080.1518916037138No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA10860.13655155764336657No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9780.12297184472855663No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9040.11366722662026092No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8810.1107752507217366No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC8740.10989508414392483No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8410.10574572741995514No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG7970.10021325178799553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10450.050.562061
GGTATCA4350.042.295911
TCAACGC14750.035.3770644
ATCAACG15000.034.4740453
TACACCG550.002713657734.1891375
CAACGCA15750.033.13095
AACGCAG16350.032.2026146
GTACATG30550.030.8845881
TACATGG30500.030.8262712
ACATGGG31500.029.9968973
ATGGGAG7250.027.8818285
GTCTTAG4450.027.5636251
TATCAAC19800.027.541252
ATAGGGT1057.407944E-526.8628943
ACCTAAG4800.026.5366171
TTAGGTA3100.025.779714
GTCCTAC1109.923829E-525.7324751
TAGGTAT2950.025.4969845
GGTACCT3900.025.3067468
CTTAGGT3550.025.1603153
Produced by FastQC (version 0.11.2)