FastQCFastQC Report
Sun 14 Apr 2019
SRR6575851_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575851_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1551808
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA22930.147763125335093No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC21020.13545490163731597No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC18390.11850692869220934No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16810.10832525673279168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22600.052.955981
GGTATCA8650.044.6670721
ATCAACG30000.039.482453
TCAACGC29900.039.45734
CAACGCA30900.037.8761375
AACGCAG31650.037.127116
GTACATG62250.031.0337491
TATCAAC38350.030.885882
TACATGG65050.029.336182
ACGCAGA41200.028.6315827
ACATGGG66150.028.564133
AGGTATA6450.027.6916436
ACCTAAG9150.027.2925741
GTCTTAG8550.027.0034791
CGCAGAG44650.026.4192878
TAGGTAT6800.026.2663365
GGTATAG7250.025.92937
CTTAGGT7050.025.3349063
TTAGGTA6900.025.2044644
CTAAGAC11250.025.0682243
Produced by FastQC (version 0.11.2)