FastQCFastQC Report
Sun 14 Apr 2019
SRR6575846_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575846_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620931
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC15890.2559060507528212No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC13960.2248236921654741No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA13350.21499973426999133No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT13240.21322820087900266No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA12990.20920198862675565No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT11260.1813405998412062No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT11200.1803743089006669No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA9610.1547675989763758No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG9360.15074138672412876No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC9250.14896985333314008No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC8900.14333315617999423No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC8590.1383406529872079No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT8310.13383129526469126No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA8180.1317376648935228No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT8030.12932193754217458No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA7300.11756539776561326No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG7090.11418337947372574No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT6920.11144555514219776No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT6860.11047926420165848No Hit
ATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCA6510.10484256704851264No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG6440.10371522761788347No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACT500.001701009337.6018684
ATGGGTA2150.030.6061735
ACCTAAG1859.094947E-1230.561841
TAACCAC1401.8872015E-830.2157865
ATACGGG4550.029.9575333
CATGGGT3350.029.4641514
TTAGGTA1005.3178435E-528.2014034
TACGGGC5000.028.20144
GTATAAG4450.027.528551
CATGGGG16350.027.3102264
GGGTACC1555.0771632E-827.289487
ATAAGGT4150.027.1820763
TGTATAC700.00883080926.8584773
TAGTACT6000.026.6346574
GTACAGG1458.4555904E-725.9951291
AGTACTT9000.025.58809912-13
TATAATA1301.0863589E-525.308952
TAAGGTG4300.025.1407854
CGGGCGT5450.025.0104186
TGGGTAC1701.241333E-724.883596
Produced by FastQC (version 0.11.2)