FastQCFastQC Report
Sun 14 Apr 2019
SRR6575843_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575843_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences922355
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA40510.43920182576123074No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC29770.32276075914371366No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC27200.2948973009307696No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21250.23038851635216376No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT18890.2048018387714058No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC15760.17086696553929887No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC14820.16067566175713258No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC14290.15492950111399625No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT13490.14625605108662065No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG13480.14614763296127847No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG12450.1349805660510324No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG12350.13389638479761046No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT11510.12478926226886611No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT11070.12001886475380955No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC10360.11232117785451373No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA10060.10906863409424787No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA9450.102455128448374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG4550.036.1554531
CGTTAAC1502.910383E-1134.4681971
TTAGGTA7400.034.2876664
AGGTATA7400.034.2876666
TAGGTAT7650.033.1671565
CTTAGGT7750.032.739193
TAGTACT5750.032.686474
CTGTGCG6200.031.8297679
ATAGACG753.2804435E-431.3245328
GTGCAAG3350.030.8670431
TCTTAGG9450.030.8373472
GTACTGT6750.030.6284336
GTATATA2000.030.5513571
CATGGGG30350.029.7247624
ATGGGGG17700.028.6699145
GTACTTG3700.027.9471871
GACGTTA856.826259E-427.6392947
CTTAGGG2750.027.3377743
AAGGGTA3800.027.2028835
ACCTAAG13150.027.1647051
Produced by FastQC (version 0.11.2)