FastQCFastQC Report
Sun 14 Apr 2019
SRR6575841_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575841_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences684026
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA14910.2179741705724636No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC14470.2115416665448389No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT13490.1972147257560385No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC11670.17060755000540914No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT10840.15847350831693532No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG8770.1282115007324283No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA8720.12748053436565276No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT8590.1255800218120364No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8260.12075564379131788No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA8250.12060945051796278No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7860.11490791285711363No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA7850.11476171958375851No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7660.11198404739001148No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC7300.10672108954922765No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA7020.1026276778952847No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA6950.10160432498179893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATAAT2600.034.3367233
CTTAGGT1405.11136E-1033.562213
TAGGTAT1551.5224941E-930.3142575
ATGGGGG10800.030.0195335
GTACAAT804.7763353E-429.3776721
TGGGTAC2400.029.3669386
TTAGGGT1303.3362812E-728.9151364
TAGTATG650.00616070728.9151367
CATGGGG16950.028.829844
ACGGTTA505.334233E-528.19225732-33
GTACTAG3700.027.948491
AGGTATA1704.081812E-927.639476
CTAACTG2053.6379788E-1127.5046444
CTATTAT2253.6379788E-1227.1580281
GCATATA909.5086667E-426.1134871
TCTTAGG2900.025.9333952
TAGGGTG1458.6626096E-725.9239185
TCTAACG2208.731149E-1125.6386972
GGGTACC2750.025.6293287
TTAGGTA1851.0051735E-825.3984324
Produced by FastQC (version 0.11.2)