FastQCFastQC Report
Sun 14 Apr 2019
SRR6575840_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575840_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740720
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC16540.22329625229506428No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA13110.17698995571876014No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT12250.16537963062965763No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11680.15768441516362458No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC10740.14499405983367533No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA9880.13338373474457285No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT9230.12460848903769306No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8440.11394319040933146No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA8170.1102980883464737No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA8140.10989307700615618No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC7860.10611297116319257No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGATA608.822721E-539.16548
GTACTAG3550.035.820281
CTTAAGA851.754331E-533.1753962
ACCTAAG2150.032.85841
TTAAGAC753.2750465E-431.3323213
TCTAACG2550.031.3323212
GTATAAG4350.029.2326431
GGGTACG650.006091082528.9827921
TAGTACT7200.028.7212944
CTAACGC2800.028.5347923
CTAGTAC8100.028.431183
CCTAGTA8300.027.7460922
TATTAAG856.815072E-427.6461642
AGTACGA856.815072E-427.6461649
GTACTGT7550.027.3898436
CTGTGCG7800.027.114519
CATGGGG17100.026.385114
CGCCTAC3050.026.1958757
GTATCAA26300.026.1451241
GTCTAAC3250.026.0845131
Produced by FastQC (version 0.11.2)