FastQCFastQC Report
Sun 14 Apr 2019
SRR6575838_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575838_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences999120
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA19030.19046761149811836No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16330.1634438305709024No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC12460.12470974457522618No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11700.11710305068460246No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG10420.10429177676355193No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10210.10218992713587959No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10080.10088878212827289No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13600.054.7647361
GGTATCA4950.048.567731
ATCAACG18100.041.5511863
TCAACGC18700.039.9646344
CAACGCA19100.039.1257175
AACGCAG19750.037.6000636
GTACATG34500.032.65591
ACGCAGA23400.031.9343537
TTAGCGT600.004159671331.3349534
TACATGG36050.031.2930582
TATCAAC24150.030.9472472
ACATGGG37300.030.370383
AGGTATA4100.028.6585836
TAGGTAT4000.028.2000455
CGCAGAG26750.027.7594038
GTCTTAG4800.027.4979081
TATAATA1904.474714E-1027.2119314
CATGGGG24050.027.1656444
TATAGTA4500.027.1542389
TATACCC700.00883109926.8598733
Produced by FastQC (version 0.11.2)