FastQCFastQC Report
Sun 14 Apr 2019
SRR6575838_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575838_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences999120
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA18030.18045880374729764No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC17260.17275202177916565No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12960.12971414845063656No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC12620.1263111538153575No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11080.11089758987909361No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10840.10849547601889661No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA10610.10619345023620787No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG10510.10519256946112579No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13400.053.3344461
GGTATCA5250.049.25751
TCAACGC17650.039.6748124
ATCAACG17900.039.1206973
CAACGCA19450.036.2447435
AACGCAG20000.035.248016
AGGTATA4800.032.3106776
TTAGGTA5250.031.3315664
TAGGTAT5100.031.3315645
GTACATG34350.030.7981621
GTATAGT5150.030.1148078
TATCAAC23800.029.8310832
TACATGG35800.029.6820852
CTTAGGT5250.029.5411913
ACGCAGA23900.029.2996047
GGTATAG5350.028.989027
GTACTAG650.006141952228.9344751
GTCTTAG6550.028.7136021
ACATGGG37400.028.6507873
TATACAG2151.8189894E-1228.4170025
Produced by FastQC (version 0.11.2)