FastQCFastQC Report
Sun 14 Apr 2019
SRR6575837_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575837_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386837
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6000.15510408776823315No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4480.11581105220028075No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGGTA250.005234560556.39689694
GTATCAA15150.034.247491
GTACATG25300.033.371921
TACATGG25350.033.004422
ACATGGG25400.032.014193
CATGGGG8850.028.6800964
AACGCAG17750.028.5993086
ATCAACG18250.028.0733183
TCAACGC18350.027.920334
CAACGCA18400.027.8444585
TTAGACC856.8049005E-427.6491133
ATGGGAG6050.027.1921025
TATCAAC19100.027.0700722
GTGTTAA1406.154896E-726.953221
GTCCTAC1301.0557365E-525.3982281
CATGGGA15650.025.2287124
ACGCAGA20350.024.9421187
ATGGGGA4550.024.7930495
CGCAGAG20950.024.6764498
GCAGAGT22500.023.185399
Produced by FastQC (version 0.11.2)