FastQCFastQC Report
Sun 14 Apr 2019
SRR6575837_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575837_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386837
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5200.13442354273246873No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4500.11632806582617486No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4240.10960688868955143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13300.039.238621
TAGTACT1055.0995368E-835.8075564
GTACATG26100.033.8656771
TACATGG26400.033.302752
AACGCAG15600.033.1392026
ATCAACG15500.033.0497933
CAACGCA15800.033.017175
TCAACGC15900.032.8095174
ACATGGG27050.031.6211813
TATCAAC16850.030.9717332
CATGGGG9400.029.9983484
ATGGGAG6300.029.8396285
ACGCAGA17500.029.5412337
GTATTAC804.7657877E-429.3847791
CCTAATA804.7657877E-429.3847792
CGCAGAG17750.029.1251588
GTCTAGC700.008814583526.8660831
CATGGGA15900.026.6023124
GCAGAGT19650.026.5481599
TATAGGG1101.0143277E-425.64492
Produced by FastQC (version 0.11.2)