FastQCFastQC Report
Sun 14 Apr 2019
SRR6575836_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575836_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1099883
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA49950.45413921298901794No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC44480.40440665052555597No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC37400.3400361674832687No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG19930.18120109138881135No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT19190.1744731030482333No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG18900.17183645896881758No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG16420.14928860615174522No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT16220.147470230924562No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC14520.13201404149350432No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA12890.1171942833919608No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC12770.11610325825565082No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA12050.10955710743779112No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT11960.10873883858555865No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA11760.10692046335837538No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT11380.1034655504267272No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG11270.10246544405177642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22350.053.5263981
GGTATCA8400.041.491991
ATCAACG29550.039.75633
TCAACGC29550.039.438254
CAACGCA29800.038.9497075
AACGCAG31250.037.443196
GTCTTAG12000.036.109241
TAGGCCG801.1599639E-535.243965
TATCAAC33400.034.8938142
GGTAATC2450.034.5514078
CTTAGGT11100.034.2914243
TTAGGTA10950.033.902874
GTACATG56000.033.8103641
TAGGTAT11600.032.8133475
AGGTATA11800.032.257196
AAGGGTA2950.031.8589535
ACATGGG57850.031.842433
TACATGG60950.030.8410762
ACGCAGA38400.030.2265957
GGTATAG12350.030.0596317
Produced by FastQC (version 0.11.2)