FastQCFastQC Report
Sun 14 Apr 2019
SRR6575830_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575830_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1155984
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13610.11773519356669297No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13330.11531301471300641No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12840.11107420171905494No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA12840.11107420171905494No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT12230.1057973120735235No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11950.10337513321983696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15700.053.690141
GGTATCA6950.039.2992551
TCAACGC21100.038.974034
ATCAACG21700.037.8964043
CAACGCA22050.037.081765
AACGCAG23350.035.2185026
TATCAAC27850.029.8679472
GTACATG43550.029.8443451
TACATGG44200.029.4519982
GTACTAG650.006096074428.9792941
ACATGGG44250.028.354223
TAGGTAT3750.027.568375
ACGCAGA30100.027.3218467
CGCAGAG30700.026.652098
CTAAGAC7600.025.9690113
GTCTTAG5200.024.4512791
GCAGAGT34500.023.7164969
GTATATG2202.397428E-923.5456771
ATGGGAG10450.023.3833545
ACCTAAG5250.023.321431
Produced by FastQC (version 0.11.2)