FastQCFastQC Report
Sun 14 Apr 2019
SRR6575830_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575830_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1155984
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13810.11946532131932623No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12790.11064166978089662No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12640.10934407396642168No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12620.10917106119115835No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA12520.10830599731484172No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA11610.10043391604036042No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17250.053.4116021
GGTATCA6750.043.8738141
TCAACGC22950.039.9238744
CAACGCA23400.039.1561055
ATCAACG23400.039.1561053
AACGCAG24050.038.097836
CTTAGGT3300.031.3248833
GTCTTAG5250.030.4430541
ACCTAAG4950.030.388791
TATCAAC31050.029.8245052
ACGCAGA30750.029.6440357
GTACATG44150.029.1734981
TACATGG44050.028.7061522
CTAAGAC7850.028.132543
CGCAGAG32750.027.8337148
ACATGGG46850.027.0791423
TTAGGTA4200.026.8499014
TAAGACA8250.026.7685384
TAGGTAT4100.026.3587445
AGGTATA3950.026.1701566
Produced by FastQC (version 0.11.2)