FastQCFastQC Report
Sun 14 Apr 2019
SRR6575829_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575829_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1267361
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15210.1200131612066333No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14840.1170937088958868No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14280.11267507837151372No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA14080.11109699604138047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18550.054.479771
TCAACGC24450.041.3284264
ATCAACG25100.040.0709233
CAACGCA25700.039.1354145
GGTATCA7000.038.9466971
AACGCAG26800.037.353746
GTACATG48100.030.7826611
TATCAAC33150.030.6274552
ACGCAGA33000.030.4781867
TACATGG49250.030.4456422
ACCTAAG5600.029.37791
TTAGGTA3850.029.2981244
ACATGGG50600.029.165433
CGCAGAG35800.028.2256988
GTACCGT1005.3246724E-528.1994446
CTAAGAC7750.027.2897853
GCAGAGT38600.026.39
TAGGTAT3700.025.4049075
CTTAGGT4350.024.8500843
GTCTTAG6150.024.4576971
Produced by FastQC (version 0.11.2)