FastQCFastQC Report
Sun 14 Apr 2019
SRR6575827_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575827_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1560577
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA21040.1348219280432814No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17490.11207393162913461No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17450.11181761617658084No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17230.11040788118753514No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16090.10310289078975275No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT16050.10284657533719899No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22300.053.461441
ATCAACG29050.039.9619983
TCAACGC29450.039.578814
CAACGCA30750.037.7527165
GGTATCA8600.037.259361
AACGCAG31950.036.923196
GTACATG63900.031.7834781
TACATGG64800.031.1156352
ACATGGG65750.030.4516073
GTCTTAG8550.030.3124871
TATCAAC38800.030.2834012
ACGCAGA39300.030.0167487
CGCAGAG42700.027.7367238
CTTAGGT4950.026.5857683
CATGGGG39000.025.3076084
GCAGAGT48400.024.7615189
ACCTAAG8550.024.2499891
TAGGTAT4950.023.7372915
GGTACCT9050.023.3693398
TTAGGTA5500.023.0726474
Produced by FastQC (version 0.11.2)