FastQCFastQC Report
Sun 14 Apr 2019
SRR6575822_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575822_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences892475
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA16020.17950082635367937No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC15690.17580324378834142No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT15500.17367433261435894No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC14700.16471049609232752No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA13990.1567550911790246No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA12510.14017199361326646No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG12270.13748284265665706No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT12220.13692260287403007No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC11740.1315443009608112No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11630.1303117734390319No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT11090.12426118378666068No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG10110.11328048404717218No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9520.10666965461217401No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9440.10577327095997087No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC9400.10532507913386929No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9240.103532311829463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAAG1359.094947E-1238.3010442
ATTAAGG2100.031.337223
ACCTAAG1551.4770194E-930.4012951
GTACTAG4250.029.9363331
TTAAGGT1802.382876E-1028.7241744
GTATAAG5500.028.2732071
TAGTACT6250.027.825894
TTAGGTA1205.8381793E-627.418534
CATGGGT4400.026.706364
CATGGGG23900.026.3532564
GTATTAA909.3730725E-426.1788921
CATAAGT909.512231E-426.1128864
GTACTGT6700.025.9569876
CTATTAT2007.876224E-1025.9171051
TATTCTC2550.025.8056775
GTATGTA1301.0658216E-525.373391
AGGTATA950.001303840724.7385236
GTGATCG5900.024.6938258
TGATCGC6100.024.6546529
ATAAGGT6500.023.8644983
Produced by FastQC (version 0.11.2)