FastQCFastQC Report
Sun 14 Apr 2019
SRR6575822_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575822_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences892475
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA16930.18969719039749014No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC16920.18958514244096472No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC15600.17479481217961287No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT15600.17479481217961287No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA14680.16448640017927674No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG14590.1634779685705482No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13120.14700691896131546No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA12710.14241295274377433No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT11530.12919129387377798No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT11490.12874310204767642No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10700.11989131348217037No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10400.1165298747864086No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC10190.11417686769937534No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT9820.11003109330793578No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC9720.10891061374268186No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA9670.1083503739600549No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9340.10465279139471695No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA9110.1020756883946329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAT1151.8189894E-1244.9543535
AGGTATA1201.8189894E-1243.0812536
CTTAGGT1400.040.283773
TATTAAG757.4327545E-637.6066132
TTAGGTA1601.8189894E-1235.24834
GGATACG600.00415690831.3388421
TAATAAT4650.031.3318213
CATGGGG24250.028.6831514
GTACTAG4150.028.3182321
TTAAGGT1354.634694E-727.8505084
CACTATC3750.027.5720027
TCTTAGG2053.6379788E-1127.5170332
TAGTACT6600.027.05934
GTCCTAC3850.026.8618661
TATATCG700.00883706126.8558484
ATGGGGG16000.026.729965
TCTACAC1956.0572347E-1026.511543
GTATCAA35650.026.503961
CTGTGCG7500.026.318739
GTACATG55000.025.3844641
Produced by FastQC (version 0.11.2)