FastQCFastQC Report
Sun 14 Apr 2019
SRR6575820_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575820_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1085944
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA19110.17597592509374332No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17570.16179471501292883No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA17390.16013717097750896No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA16530.1522177939193918No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC16330.15037607832448083No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA15640.14402215952203798No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14390.13251143705384438No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT13970.12864383430453136No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT13790.12698629026911148No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG13430.12367120219827174No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13120.12081654302615974No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13080.12044819990717753No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG12640.11639642559837339No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC12570.11575182514015456No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA12130.11170005083135043No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC11760.10829287698076512No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG11760.10829287698076512No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG11730.10801661964152846No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT11520.10608281826687195No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA10890.1002814141429024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAAC2000.037.603391
CTGTGCG7550.029.8807379
GTATATA1802.382876E-1028.7248121
CCTAGTA7250.027.8783742
TAGTACT7100.027.8027634
TCTAGAC856.8159204E-427.6470053
CTAGTAC7500.027.573283
TAACCAC2053.6379788E-1127.512146
TATTAAG1807.490598E-926.1134662
ACCTAAG3250.026.0331151
GTACTAG3450.025.8863931
TAATAAT4200.025.7380453
GTACTGT8250.025.6363146
TACATGG58200.025.52122
ACATGGG57100.025.4342753
GTATAAG5550.025.4076961
GTACATG59250.025.3069231
GTATCAA34050.025.2621881
CATGGGG29750.024.9612964
GTTAATA4900.024.9410231
Produced by FastQC (version 0.11.2)