FastQCFastQC Report
Sun 14 Apr 2019
SRR6575817_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575817_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642475
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA15370.23923109848632243No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT14400.22413323475621616No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC10320.16062881824195496No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9080.14132845636016964No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC9050.1408615121210942No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT8680.13510253317249699No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA8090.1259192964706798No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT7900.12296198295653528No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG7570.11782559632670532No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG7290.11346745009533446No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA7070.11004319234211447No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT7000.10895365578427177No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC6990.10879800770457995No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT6890.10724152690766177No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT6670.10381726915444181No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACA250.005208974756.471041
GTATAAG4400.034.2248731
GGGTACC2800.033.566627
GTACTAG3250.033.3034321
TCAGTAG851.7562372E-533.1691363
CTTAGGG1457.4396667E-1032.4066283
TATTCTC2250.031.3288465
TAAGGTG4150.029.4392744
TCTTAGG2101.8189894E-1229.0888062
ATAAGGT4200.029.0888063
TAGGAGT650.006158823628.9166834
TGATCGC4400.028.8547469
CATGGGT4750.028.6883954
ATGGGGG10000.027.726035
TATTAAG856.821604E-427.6409472
GTGATCG4600.027.6001918
CATGGGG14850.027.5292664
TGGGTAC3550.026.475086
ATGGGTA3600.026.107375
TAGAAGG909.525376E-426.105342
Produced by FastQC (version 0.11.2)