FastQCFastQC Report
Sun 14 Apr 2019
SRR6575817_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575817_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642475
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA16440.2558854430133468No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT15330.23860850616755513No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC11100.17276936845791666No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC10290.16016187400287948No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG9010.14023891980232694No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8780.13665901396941516No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT8420.13105568310050975No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA8120.12638624070975524No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT8020.12482975991283708No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA7850.1221837425580762No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7840.12202809447838436No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG7440.1158021712907117No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7360.11455698665317716No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA7090.11035448850149812No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT7060.10988754426242266No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT6570.10226078835752364No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6530.10163819603875637No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA6430.10008171524183819No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTGA2850.037.9175459
ACCGTGC1152.7775968E-936.7735678
AGGGCTA3550.034.414075
GGGCTAT3500.033.563176
ATTTAGG4100.030.9436071
GGCTATT3700.030.4789897
GTATATA1005.328851E-528.1930681
ATGGGTA3700.027.9390725
CTAGTAC5650.027.4445763
GTATAAG3300.027.0539511
TTATATA700.00884377226.850544
GTCCTAC1956.0572347E-1026.50631
TAGTACT5000.026.3135284
GTTTAAT909.5267565E-426.104691
GTACTAC909.5267565E-426.104691
CGTTAAC1458.6590626E-725.9246561
CGCAAGT2550.025.79757776-77
GGGTACC3650.025.7470937
TTTAGGG4750.025.721992
CCTAGTA6050.025.6320552
Produced by FastQC (version 0.11.2)