FastQCFastQC Report
Sun 14 Apr 2019
SRR6575816_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575816_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672352
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA14070.20926538479843892No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT13840.20584455761267906No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC11780.17520584455761268No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC10690.15899409832944647No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG9950.1479879586883061No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9570.14233615725096377No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT9050.1346021131788111No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA8950.13311479701108944No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8310.12359597353767074No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT8250.12270358383703775No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA8190.12181119413640475No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG8040.11958021988482223No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA7820.11630812431583457No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT7550.11229237066298606No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7470.11110251772880873No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT7110.10574817952501071No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC6890.10247608395602303No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA250.00170232937.59910610-11
ACCTAAG1451.8189894E-1135.6622851
GTACTAG3000.031.3395821
TCTCGTG650.006153090428.9223885
ACGCAAG1154.19376E-628.6080178
CCTAAGA2151.8189894E-1228.424272
AACGCAA1205.8395326E-627.4160147
CGTTTGA350.008837569526.85650458-59
TTAATAA3550.026.4841542
TAATAAT3600.026.110493
GTTAATA3700.025.4104731
ATCAGGC8450.025.0289928
GTATAAA9800.024.943751
TATAAAA9050.024.9331462
TAGGCGG950.00130420324.7362545
GTATCAA23650.024.647621
CTAACGC3250.024.5840323
TACGGGC5400.024.3697914
TAAGGTG3300.024.2115464
CATGGGG16850.023.987564
Produced by FastQC (version 0.11.2)