FastQCFastQC Report
Sun 14 Apr 2019
SRR6575814_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575814_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312393
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10440.3341944281722062No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5400.17285918698562386No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5180.16581677566398734No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5000.16005480276446654No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4850.15525315868153255No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4690.15013140499306962No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4330.13860745919402803No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC4080.1306047190558047No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4080.1306047190558047No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3960.12676340378945752No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA3930.12580307497287072No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3660.11716011562358951No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3620.11587967720147378No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3590.11491934838488697No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3590.11491934838488697No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3550.11363890996277126No Hit
CGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCC3490.11171825232959766No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3420.10947748509089511No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG3370.10787693706325045No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG3150.10083452574161393No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC3130.10019430653055605No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAC250.00523325656.397898
TACCGAT250.00523325656.397897
GTATATG751.4382022E-744.0129781
GTATCAA8800.040.7262951
ATAATAC757.4281324E-637.5985953
GTACATG16950.034.4981651
TACATGG16550.034.3667532
CATATAC550.002714492434.180543
TCAACGC10350.034.0566944
GGGTACC3600.033.9431727
CAACGCA10450.033.7307975
ATCAACG10500.033.5701753
AACGCAG10750.033.226666
ACATGGG16900.033.0934333
TGGGTAC4150.031.7096566
TATATGA753.2677728E-431.3371772
CATGGGG8500.030.9635454
TATAATA953.7404963E-529.6878492
GGTACCT4200.029.0941478
GCCCTAA650.00604817729.0195471
Produced by FastQC (version 0.11.2)